Polymerase Chain Reaction ​
Tags
Cegep1
Biology
Word count
134 words
Reading time
1 minute
DNA amplification (mass replication) technique in vitroAbbr. PCR
Advantages ​
- Quick
- Targets specific sequence
- Requires a small amount of DNA
Materials ​
- DNA
- Primers: short pieces of single stranded DNA complementary to either end of the template DNA
- Deoxynucleoside triphosphates (
abbr.dNTPs): precursors- dATP, dGTP, dTTP, dCTP
- Taq polymerase
Steps ​
- Combine the materials in an eppendorf.
- Cycle:
- Denaturation: the two strands of DNA are separated at high temperature.
- 95°C
- Annealing: primers bind to their complementary sequences of DNA.
- 55°C
- Polymerization: Taq polymerase synthesizes complementary DNA.
- 72°C
- Denaturation: the two strands of DNA are separated at high temperature.
- Repeat the cycle for exponential amplification.
[!abstract] Eppendorf
Small tube
A.k.a. microcentrifuge tube
Ajitani Hifumi