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Hifumi's Study Notes📕Cegep 1BiologyPolymerase Chain Reaction

Polymerase Chain Reaction ​

Tags
Cegep1
Biology
Word count
134 words
Reading time
1 minute

DNA amplification (mass replication) technique in vitro
Abbr. PCR

Advantages ​

  • Quick
  • Targets specific sequence
  • Requires a small amount of DNA

Materials ​

  • DNA
  • Primers: short pieces of single stranded DNA complementary to either end of the template DNA
  • Deoxynucleoside triphosphates (abbr. dNTPs): precursors
    • dATP, dGTP, dTTP, dCTP
  • Taq polymerase

Steps ​

  • Combine the materials in an eppendorf.
  • Cycle:
    • Denaturation: the two strands of DNA are separated at high temperature.
      • 95°C
    • Annealing: primers bind to their complementary sequences of DNA.
      • 55°C
    • Polymerization: Taq polymerase synthesizes complementary DNA.
      • 72°C
  • Repeat the cycle for exponential amplification.

[!abstract] Eppendorf
Small tube
A.k.a. microcentrifuge tube

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